Chronic lymphocytic leukemia (CLL) is a chronic B lymphocyte proliferative disorder characterized by the accumulation of monoclonal and mature small lymphocytes in peripheral blood, bone marrow and lymphoid tissues, and the corresponding clinical symptoms. CLL is common in Western countries, accounting for 65% of leukemia patients over 65 years of age. The incidence rate in the Asia-Pacific region is relatively lower. The median age of onset was 65 to 70 years old. The disease is more common in men, and the ratio of male to female cases is about 2 to 1. The cause of the disease is not fully understood, and environmental factors do not predominate in the pathogenesis of chronic lymphocytic leukemia.
Studies have reported that CLL-related gene variants, not only contain the mutations in TP53 and ATM genes, which are commonly found in many other types of cancer, but also include some new somatic mutations, such as mutations in NOTCH1, SF3B1, XPO1, MYD88, POT1, CHD2, BIRC3, FBXW7 and DDX3X. TP53 encodes a P53 protein, a tumor suppressor that regulates cell division. ATM is involved in DNA repair and cell division, and mutations in this gene result in increased susceptibility to a variety of cancers. NOTCH1 is a tumor protooncogene that encodes a Notch signaling pathway receptor protein, which is involved in cell differentiation, proliferation and survival. The activation mutation of NOTCH1 is associated with CLL. The SF3B1 gene encodes the cleavage factor 3b protein complex subunit 1, which is one of the components of the small nuclear ribonucleoprotein complex. In CLL cases, mutations in the NOTCH1 and SF3B1 genes were detected at a high frequency. Moreover, it has been reported that the variation of NOTCH1 and TP53 abnormalities are often mutually exclusive.
CD-Genomics provides a custom CLL panel platform with amplicon sequencing technology by Illumina MiSeq system/Ion PGM system to help verify the biological effects of the genetic mutations associated with CLL and to research how they affect cellular fitness. From our custom CLL panel, you can select genes which you are interested in, and you can add new genes which are not included in the panel.
For more information about the Custom CLL Panel or need other amplification requirements, please contact us.