Pan-Cancer Panel Sequencing

Description of Pan-Cancer Panel

Pan-Cancer Panel is a predesigned panel for targeted resequencing assay utilizing amplicon sequencing technology by Illumina system, designed to detect somatic variants and assessing tumor mutational burden (TMB) in tumor samples. The panel covers a total of 516 genes associated with major solid tumor types and maintains >96% correlation with whole exome datasets. CD Genomics utilizes targeted NGS sequencing technology to provide more efficient and accurate targeting of specific genes or mutations, and even to detect low frequency variations in cancer-associated genes. Targeted NGS sequencing facilitates sequencing of a large number of genes and samples in a single and cost-effective assay.

CD Genomics provides a pan-cancer panel sequencing and bioinformatics analysis for your cancer related researches.

Gene List of Pan-Cancer Panel

ABL1	ABL2	ACVR1	ACVR1B	AGO2	AKT1	AKT2	AKT3	ALK	ALOX12B
AMER1	ANKRD11	APC	AR	ARAF	ARFRP1	ARID1A	ARID1B	ARID2	ARID5B
ASXL1	ASXL2	ATM	ATR	ATRX	AURKA	AURKB	AXIN1	AXIN2	AXL
B2M	BABAM1	BAP1	BARD1	BBC3	BCL10	BCL2	BCL2L1	BCL2L11	BCL2L2
BCL6	BCOR	BCORL1	BIRC3	BLM	BMPR1A	BRAF	BRCA1	BRCA2	BRD4
BRIP1	BTG1	BTK	CALR	CARD11	CARM1	CASP8	CBFB	CBL	CCND1

(Check the Pan-Cancer Panel Sequencing Gene List for more genes.)

Highlights of Pan-cancer Panel Sequencing Service

Target-enrichment sequencing by Illumina provides extremely high depth (average depth > 1000x) with lower costs.
High coverage uniformity ensures the sequencing data more efficiency.
SNVs, indels, and even low frequency variations can be detected.
Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
Fast turnaround time.

Applications of Pan-cancer Panel Sequencing

Cancer related mechanism studies
Biomarker discovery
Therapeutic target discovery
Drug target discovery
Discover rare mutations and detect low frequency mutations

Service Specifications

Sample Requirements

Sample types for genome DNA from tissues, whole blood, circulating cells, cultured cells, and formalin-fixed paraffin-embedded (FFPE) tissues.
Recommended amount: 2μg.
Minimum amount: 200ng.
Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.

Sequencing

Illumina PE150
Enrichment Method: Amplicon sequencing
More than 80% of bases with a ≥ Q30 quality score
Recommended sequencing depth: ≥ 1000x

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

Read alignment
Variant calling/Annotation
Tumor mutation burden (TMB) analysis

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective pan-cancer panel sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for pan-cancer panel sequencing is outlined below.

Gene Panel Workflow

For more information about the pan-cancer panel or need other amplification requirements, please contact us.

References:

Zhen W X, et al. Assessment of tumor mutation burden calculation from gene panel sequencing data. oncotarget and therapy, 2019. 12, 3401—3409.
Miller, E M, et al. Development and validation of a targeted next generation DNA sequencing panel outperforming whole exome sequencing for the identification of clinically relevant genetic variants. Oncotarget, 2017. 8(60),102033-102045.

* For Research Use Only. Not for use in diagnostic procedures.