CD Genomics is a professional sequencing company with extensive experience in detecting genetic mutations. We can provide a variety of technologies for you to choose to accelerate your research on disease research, biomarker discovery, targeted drug development and more. Combining the characteristics of each technology, we can detect SNPs, indels, copy number variations, or DNA methylation.
CD Genomics offers the following four non-NGS mutation detection services, including MassARRAY, Mulitiplex Snapshot, MLPA, and ARMS-PCR. You can tell us which genes or gene loci need to be measured to customize your own mutation detection panel.
We can also use NGS technology to detect mutations in target genes. Learn about our Custom NGS Panel.
| Types | Target NGS Sequencing | MassARRAY | Mulitiplex Snapshot | MLPA | ARMS-PCR |
|---|---|---|---|---|---|
| Detection type |
|
|
SNPs |
|
Detection of known SNPs |
| Throughput | High-throughput | Medium to high throughput | Medium-throughput | High-throughput | Low-throughput |
| More suitable for targeted sequencing of more than 20 samples | The method allows multiple tests of 384 samples on a single chip, up to 40 reactions per well, and up to 100,000 genotypes per day | Interrogates up to 30 SNPs simultaneously in a single reaction | A single reaction can detect copy number changes of 45-50 nucleic acid sequences, and 96 samples can be processed simultaneously | Detection of 1 SNP site in a single reaction | |
| Characteristics | Detects unknown sequences and more new mutations, effectively detecting low-frequency mutations | No minimum sample size required for a single test; cheap, no need to order special fluorescent probes; flexible and efficient | Fast and cheap; experiments can be supplemented at a lower cost | Large genome rearrangements can be detected; Often in combination with other techniques to detect genes | Fast; Homozygotes and heterozygotes can be detected; It is widely used to detect single gene point mutation in sickle cell anemia and thalassemia |
| Price | +++* | + | + | + | + |
* A large sample size makes the price more economical.
| Types | Target NGS Sequencing | MassARRAY | Mulitiplex Snapshot | MLPA | ARMS-PCR |
|---|---|---|---|---|---|
| Sample types |
|
|
|
|
|
| DNA Concentration | ≥20 ng/ul | 20-50 ng/µL | ≥20 ng/ul | ≥20 ng/ul | 20-50 ng/µL |
| DNA Volume | ≥15ul | ≥15 µL | ≥50ul | ≥50ul | ≥15 µL |
| Total DNA | ≥1μg | ≥1μg | ≥1μg | ≥1μg | ≥1μg |
| Blood Sample | ≥1mL whole blood | ≥0.5mL whole blood | ≥0.5mL whole blood | ≥0.5mL whole blood | ≥0.5mL whole blood |
| Tissue Sample | - | Tissue volume ≥100mg | Provide enough material to extract more than 2ug of DNA | Tissue volume ≥100mg | Tissue volume ≥100mg |
| Culture Cells In Vitro | - | ≥106 cells | ≥106 cells | ≥106 cells | ≥106 cells |
| DNA Purity | OD260/280 = 1.7~2.0, no degradation or RNA contamination | OD260/280 = 1.7~2.0, no degradation or RNA contamination | OD260/280 = 1.7~2.0, no degradation or RNA contamination, no PCR inhibitors | OD260/280 = 1.7~2.0, no degradation or RNA contamination, no PCR inhibitors | OD260/280 = 1.7~2.0, no degradation or RNA contamination |
| Preservation Methods | Low temperatures during transportation | Low temperatures during transportation | Low temperatures during transportation | Low temperatures during transportation | Low temperatures during transportation |
Want to know more about non-NGS Panel? Please contact us, we will be happy to get in touch with you.
