Pathogens Capture Sequencing (tNGS)

Description

Rapid and accurate retrieval of whole genome sequences of human pathogens from disease vectors or animal reservoirs will enable fine-resolution studies of pathogen epidemiological and evolutionary dynamics. However, next generation sequencing technologies have not yet been fully harnessed for the study of vector-borne and zoonotic pathogens, due to the difficulty of obtaining high-quality pathogen sequence data directly with a high ratio of host to pathogen DNA.

Based on targeted NGS (tNGS) technology, Our Pathogen Targeted NGS Sequencing Services harnesses the power of cutting-edge sequencing technologies to address these challenges. This sophisticated technique enriches dozens to hundreds of known pathogenic microorganisms, along with their virulence and resistance genes, using ultra-multiplex PCR amplification or hybridization capture technology. Subsequently, high-throughput multi-pathogen parallel testing, grounded in NGS, is performed.

CD Genomics tNGS Technology

Highlights of Pathogens Capture Sequencing Service

Comprehensive Coverage: Our service encompasses over 95% of current clinical pathogens, both DNA and RNA-based.
Host Background Independence: Unlike some traditional methods, our detection isn't influenced by the host background, ensuring precise results.
Enhanced Specificity: Through our target capture strategy, we achieve higher specificity in pathogen detection.
Cost-Effectiveness: Our service minimizes costs while maximizing the depth and breadth of pathogen identification. And we offer a quick turnaround time.
Diverse Microorganism Detection: 500-50000 kinds of microorganisms captured all at once including bacteria, viruses and disease-causing parasites.
Quality Control: Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.

Applications of Pathogens Capture Sequencing

Study the mechanism of pathogen infection
Resistance-related mutation detection
Genotyping
Help make treatment strategies

Service Specifications

Sample Requirements

Sample types for genomic DNA from whole blood.
Recommended amount: 2μg
Minimum amount: 1μg
Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.

Sequencing

Illumina PE150
Enrichment Method: Hybridization capture
More than 80% of bases with a ≥ Q30 quality score
Recommended sequencing depth: ≥ 500x

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

Read alignment
Variant calling/Annotation
Genotyping

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective pathogens capture sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for pathogens capture sequencing panel is outlined below.

Gene Panel Workflow

For more information about the pathogens capture or need other amplification requirements, please contact us.

CD Genomics tNGS Programs

- Multiple Pathogen Targeted NGS Service
  - CD Genomics Multiple Pathogen Targeted NGS service offers a cutting-edge solution for accurate identification of a wide range of microorganisms commonly found in the bloodstream, respiratory, and nervous systems. This comprehensive approach allows for the simultaneous detection of 219 pathogens, including elusive ones such as Mycobacterium avium, Nocardia, and Chlamydia psittaci, along with common drug resistance genes. Our service is characterized by exceptional clinical coverage, heightened sensitivity, unparalleled specificity, and the capability for precise typing. By swiftly targeting pathogens and providing crucial drug resistance information, our service stands at the forefront of diagnostic innovation.
    
    Product Advantages
    
    Broad Coverage: Drawing from a wealth of testing data, our selection of 219 pathogens and 828 genes/genotypes addresses over 95% of clinical requirements. This expansive coverage ensures a holistic approach to pathogen detection and characterization.
    
    High Sensitivity: Leveraging multiple pairs of primers for individual pathogens, we have significantly elevated sensitivity levels, enabling precise determination of pathogen types. This enhanced sensitivity empowers accurate identification and classification.
    
    Good Specificity: Our service employs a sophisticated control mechanism to ensure primer specificity. The PCR reaction system is meticulously optimized to curtail non-specific amplification of short fragments, thereby upholding exceptional amplification specificity.
    
    High Detection Rate: Through a three-pronged approach involving efficient extraction and innovative techniques, our service achieves unparalleled detection rates for fungi and intracellular bacteria. The triple wall-breaking methodology contributes to breakthrough outcomes in detection.
- Mycobacterium Targeted NGS Program
  - Our Mycobacterium Targeted Sequencing Program is tailored to meet the specific challenges posed by Mycobacterium species. The program encompasses the forward enrichment and sequencing of target genes. This precision-driven initiative allows for accurate typing and classification, supported by concurrent testing of drug-resistant genes/subtypes. The outcome is a molecular diagnostic foundation for informed decisions.
    
    Please refer to our Respiratory Pathogen NGS Panels, for more information about the Bacteria Targets of our tNGS products.
    
    Product Advantages
    
    Comprehensive Coverage: The program's scope spans 7 types of M. tuberculosis, 66 types of Nontuberculous mycobacteria (NTM), and Mycobacterium leprae. This comprehensive coverage addresses a broad spectrum of pathogenic Mycobacterium species, aligning seamlessly with clinical requirements.
    
    Accurate Drug Resistance: Through an optimized ultra-multi-targeted amplification system, we assess 1,000 loci encompassing 35 drug resistance genes. This robust approach furnishes precise and reliable drug resistance information, empowering informed treatment strategies.
    
    Enhanced Sensitivity: Our program employs an ultra-multiplex primer design that augments sensitivity in detection. This heightened sensitivity enables the accurate quantification of pathogens, enriching diagnostic precision.

Reference:

Clark S A, et al. Targeted DNA enrichment and whole genome sequencing of Neisseria meningitidis directly from clinical specimens[J]. International Journal of Medical Microbiology, 2018. 308.

* For Research Use Only. Not for use in diagnostic procedures.