Cancer Hotspot Panel Sequencing

Description

Cancer is induced by the accumulation of harmful DNA mutations in cells. At present, the mechanisms of inducing abnormal cell proliferation, differentiation and cancer production are mainly studied in three categories: activating dominant transforming proto-oncogenes by translocations and mutations, inactivating tumor suppressor gene by mutations, and disordering the DNA repair genes.

CD Genomics provides a predesigned cancer hotspot gene panel sequencing utilizing amplicon sequencing technology by illumina system, which covers a total of 65 common proto-oncogenes and tumor suppressor genes associated with the common human cancer, such as breast cancer, lung cancer and colorectal cancer. And also, you can choose the genes from the gene list to customize your exclusive sequencing and bioinformatics analysis for researches.

Gene List of Cancer Hotspot Panel

ABL1	AKT1	ALK	APC	ATM	BRAF	BRCA1	BRCA2	CDH1	CDKN2A
CSF1R	CTNNB1	DDR2	DNMT3A	EGFR	ERBB2	ERBB3	ERBB4	EZH2	FBXW7
FGFR1	FGFR2	FGFR3	FLT3	FOXL2	GNA11	GNAQ	GNAS	HNF1A	HRAS
IDH1	IDH2	JAK2	JAK3	KDR	KIT	KRAS	MAP2K1	MET	MLH1
MPL	MSH6	MTOR	NF1	NF2	NOTCH1	NPM1	NRAS	PDGFRA	PIK3CA
PIK3R1	PTCH1	PTEN	PTPN11	RB1	RET	SMAD4	SMARCB1	SMO	SRC
STK11	TERT	TP53	TSC1	VHL

Highlights of Cancer Hotspot Panel Sequencing Service

Target-enrichment sequencing by Illumina provides extremely high depth (average depth > 1000x) with lower costs.
High coverage uniformity ensures the sequencing data more efficiency.
SNVs, indels, and structural rearrangement can be detected.
Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
Fast turnaround time.
Provide a list of genes related to cancer hotspots, you can add or delete the genes according to your needs.

Applications of Cancer Hotspot Panel Sequencing

Susceptibility screening and cancer risk assessment
Therapeutic target discovery
Drug target discovery
Discover rare mutations and detect low frequency mutations

Service Specifications

Sample Requirements

Sample types for genomic DNA from tissues, whole blood, saliva, formalin-fixed paraffin-embedded (FFPE) tissues.
Recommended amount: 2μg
Minimum amount: 200ng
Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.

Sequencing

Illumina PE150
Enrichment Method: Amplicon sequencing
More than 80% of bases with a ≥ Q30 quality score
Recommended sequencing depth: ≥ 5,000x

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

Read alignment
Variant calling/Annotation
Gene expression profiling

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective cancer hotspot panel sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for cancer hotspot panel sequencing is outlined below.

Gene Panel Workflow

For more information about the cancer hotspot panel or need other amplification requirements, please contact us.

Reference:

Claudio B MD, et al. Young investigator challenge: Can the Ion AmpliSeq Cancer Hotspot Panel v2 be used for next‐generation sequencing of thyroid FNA samples? Cancer CytopathologyVolume, 2016. 124, 776-784.

* For Research Use Only. Not for use in diagnostic procedures.