Hereditary Cancer Panel Sequencing

Description

Hereditary cancer panel is a predesigned panel for targeted resequencing assay utilizing amplicon sequencing technology by Illumina system, designed to detect inherited mutations and not suitable for the detection of other types of mutations in acquired cancers. The panel covers a total of 37 genes associated with cancers of the breast, ovary, uterus, prostate, gastric and colorectal, etc.

CD Genomics utilizes targeted NGS sequencing technology to provide more efficient and accurate targeting of specific genes or mutations in hereditary cancer-associated genes. Targeted NGS sequencing facilitates sequencing multiple genes and samples in a single and cost-effective assay.

Gene List of Hereditary Cancer Panel

Exon:

APC	BARD1	BMPR1A	CDKN2A	CHEK2	EPCAM	FAM175A	GREM1	MEN1	MITF
MLH1	MRE11A	MSH2	MSH6	MUTYH	NBN	PALB2	POLD1	POLE	RAD51C
RNF139

Coding Sequence (CDS):

ATM	BAP1	BLM	BRCA1	BRCA2	BRIP1	CDH1	CDK4	PMS2	PTEN
RAD50	RAD51D	SMAD4	STK11	TP53	XRCC2

Highlights of Hereditary Cancer Panel Sequencing Service

Target-enrichment sequencing by Illumina provides extremely high depth (average depth > 1000x) with lower costs.
High coverage uniformity ensures the sequencing data more efficiency.
SNVs, indels, and structural rearrangement can be detected.
Strict quality control throughout the pipeline workflow to ensure the accuracy and repeatability of the sequencing.
Fast turnaround time.

Applications of Hereditary Cancer Panel Sequencing

Cancer risk assessment
Personalized health management plan
Therapeutic target discovery
Drug target discovery

Service Specifications

Sample Requirements

Sample types for genomic DNA from whole blood, tissues, saliva, formalin-fixed paraffin-embedded (FFPE) tissues.
Recommended amount: 2μg
Minimum amount: 200ng
Collection: DNA samples are stored in TE buffer or equivalent. Blood is collected by routine blood collection kits.

Sequencing

Illumina PE150
Enrichment Method: Amplicon sequencing
More than 80% of bases with a ≥ Q30 quality score
Recommended sequencing depth: ≥ 500x

Bioinformatics Analysis

We provide customized bioinformatics analysis including:

Read alignment
Variant calling/Annotation

Gene Panel Workflow

CD Genomics provides the accurate and cost-effective hereditary cancer panel sequencing and bioinformatics analysis. Our professional expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for hereditary cancer panel sequencing is outlined below.

Gene Panel Workflow

For more information about the hereditary cancer panel or need other amplification requirements, please contact us.

Reference:

Li J, et al. Germline mutations in 40 cancer susceptibility genes among Chinese patients with high hereditary risk breast cancer[J]. International Journal of Cancer, 2019. 144, 281-289.

* For Research Use Only. Not for use in diagnostic procedures.