Details
| Product Infomation | |
|---|---|
| Product Description: | The CD KRAS and BRAF RT-PCR Kit is a test product based on real-time PCR technology, which can detect KRAS exons 2, 3, and 4 and BRAF V600E mutations. KRAS and BRAF mutations affect the RAS-RAF-MEK-ERK signaling pathway to regulate cell growth, differentiation, and survival. KRAS and BRAF mutations are typical hallmarks of many types of cancer. |
| Features: | Exon Mutation Nucleotide Change Amino Acid Change COSMIC ID 2 G12D c.35G>A Glycine (G) to Aspartic acid (D) 521 G12C c.34G>T Glycine (G) to Cysteine (C) 516 G12S c.34G>A Glycine (G) to Serine (S) 517 G12R c.34G>C Glycine (G) to Arginine (R) 518 G12A c.35G>C Glycine (G) to Alanine (A) 522 G12V c.35G>T Glycine (G) to Valine (V) 520 G13D c.38G>A Glycine (G) to Aspartic acid (D) 532 3 Q61H c.183A>C & c.183A>T Glutamine (Q) to Histidine (H) 554 & 555 Q61L c.182A>T Glutamine (Q) to Leucine (L) 553 Q61R c.182A>G Glutamine (Q) to Arginine (R) 552 4 K117N c.351A>C & c.351A>T Lysine (K) to Asparagine (N) 19940 & 28519 K117R c.350A>G Lysine (K) to Arginine (R) 1178068 K117E c.349A>G Lysine (K) to Glutamic acid (E) 1360831 A146T c.436G>A Alanine (A) to Threonine (T) 19404 A146P c.436G>C Alanine (A) to Proline (P) 19905 A146V c.437C>T Alanine (A) to Valine (V) 19900 |
| Sequencing Platform: | Real-time PCR |
| Storage: | Store in < -20℃ freezer. Repeated thawing and freezing should be avoided. |
| Method: | Real-time qualitative PCR |
| Equipment Required: | Real-time PCR instrument (FAM and VIC) |
| Required Reagents: | DNA extraction kit, pipettors and filter tips |
| ShippingCondition: | Dry ice |
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