Details
| Product Infomation | |
|---|---|
| Product Description: | The CD BRAF Codon 600 RT-PCR Kit is an assay product that uses specific probes to identify BRAF V600E, V600K, V600D, V600R, V600M, and V600G mutations. The RAF gene encodes a serine/threonine protein kinase that plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell growth and spreading. Mutations in BRAF have been found in cancers including non-Hodgkin's lymphoma, colorectal cancer, malignant melanoma, papillary thyroid carcinoma, non-small cell lung cancer, and lung adenocarcinoma. And BRAF mutations are generally found in tumors with wild-type KRAS, NRAS, and EGFR. |
| Features: | Detects as little as 1% mutation load. Highly sensitive and specific. Works with FFPE, fresh frozen, FNA and plasma samples. Includes internal control. Available as RUO and CE-IVD. Mutation Name Nucleotide Change Amino Acid Change Frequency V600E c.1799T>A Valine (V) to glutamic acid (E) 70-90% V600K c.1798_1799GT>AA Valine (V) to lysine acid (K) 10-15% V600D c.1799_1800TG>AT Valine (V) to aspartic acid (D) <5% V600R c.1798_1799GT>AG Valine (V) to arginine (R) <5% V600M c.1798G>A Valine (V) to methionine (M) <1% V600G c.1799T>G Valine (V) to Glycine(G) <1% |
| Sequencing Platform: | Real-time PCR |
| Storage: | Store in < -20℃ freezer. Repeated thawing and freezing should be avoided. |
| Method: | Real-time qualitative PCR |
| Equipment Required: | Real-time PCR instrument (FAM and VIC) |
| Required Reagents: | DNA extraction kit, pipettors and filter tips |
| ShippingCondition: | Dry ice |
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