Details
Product Infomation | |
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Product Description: | CD Tumor Structural Variation 5% gDNA Standard is a highly characterized, biologically relevant quality control material covering multiple genetic variants. This standard has been validated by ddPCR for 8 common structural variant loci in the cancer genome, with variant types including copy number variation/amplification, translocation and large fragment insertion/deletion, and insertion/deletion within high/low GC regions. |
Features: | Quality Standards (1)Allele Frequency (AF) Genotype is tesed by Droplet Digital PCR, the acceptance criteria as follows: AF=0%, acceptable range ≤ 0.1%; AF < 5%, acceptable margin of error = ± 30%; 5% ≤ AF < 10%, acceptable margin of error = ± 20%; 10%≤AF, acceptable error range = ±10%. (2)Integrity is tesed by Agarose gel electrophoresis, the acceptance criteria as follows: Bright band of high molecular weight. SNV: AKT1 E17K,PIK3CA E545K,. Deletion: EGFR ΔE746_A750. Insertion: EGFR A767_V769dup. Fusion: CD74(6)-ROS1(34),EML4(6)-ALK(20). CNV: MET Amplification, ERBB2 Amplification. |
Method: | Quality Control Method: ddPCR |
Concentration: | 30 ng/μL |
Application: | CD Tumor Structural Variation 5% gDNA Standard is used to evaluate the performance of the NGS process, it helps to revise and monitor your experimental protocols, operational specifications and bioinformatics processes, etc., and to perform a range of subsequent applications. |
Preservation Solvent: | Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0 |
Storage: | Store at 2-8℃, 36 months. |
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