Details
| Product Infomation | |
|---|---|
| Product Description: | The CD Lung Cancer ctDNA Standard Kit is a highly characterized, biologically relevant quality control material. Covering multiple genetic variants, it contains 6 SNV, 1 insertion and 1 deletion variant loci validated by ddPCR, and each loci contains 0%, 0.1% and 1% mutation frequencies in three gradients, which can be used for minimum detection limit validation and linearity validation. Assists in the performance evaluation of NGS processes, revision and monitoring of your experimental protocols, operational specifications and bioinformatics processes, among other applications. |
| Features: | Quality Standards (1)Allele Frequency (AF) Genotype is tesed by Droplet Digital PCR, the acceptance criteria as follows： AF=0%, acceptable range ≤0.1% AF <1%, acceptable error range = ±50% (the AF value detected at the 0.1% locus must be at least 0.05% higher than the corresponding wild-type locus) 1% ≤ AF <5%, acceptable error range = ±40% 5% ≤ AF < 10%, acceptable margin of error = ± 30% 10% ≤ AF, acceptable margin of error = ±20% Copy number <5, acceptable error range = ±40% 5 ≤ copy number <10, acceptable error range = ±30%. (2)Fragment distribution is tesed by 4150 TapeStation System, the average fragment length 160bp±10%. SNV: EGFR L858R, EGFR T790M, KRAS A146T, KRAS G12D, NRAS Q61K, PIK3CA E545K. Insertion: EGFR_A767_V769dup. Deletion: EGFR ΔE746_A750. |
| Method: | Quality Control Method: ddPCR |
| Concentration: | 20 ng/µL |
| Application: | CD Tumor SNV Wild Type ctDNA Standard is suitable for all processes of liquid biopsy: from library preparation, variant detection, to information analysis; and also for all assays: NGS sequencing, PCR detection, etc. |
| Preservation Solvent: | Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0 |
| Storage: | Store at 2-8℃, 24 months. |
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