Targeted Next Generation Sequencing (tNGS) for Pathogen Detection

In the realm of infectious diseases, accurate identification of pathogens plays a pivotal role in determining the most effective treatment and management strategies. The complexity of pathogenic microorganisms, coupled with the limitations of traditional clinical methods such as low throughput and time-consuming culture techniques, often result in the challenge of diagnosing pathogens in approximately 50% of infected patients. Enter the realm of innovation: the application of high-throughput sequencing technology, also known as Next Generation Sequencing (NGS), is revolutionizing pathogen detection.

Limitations in Traditional Pathogens Testing

Enhancing Precision Anti-Infection Strategies Through tNGS Technology

Based on targeted NGS (tNGS) technology, Our Pathogen Targeted NGS Products harness the power of cutting-edge sequencing technologies to address these challenges. This sophisticated technique enriches dozens to hundreds of known pathogenic microorganisms, along with their virulence and resistance genes, using ultra-multiplex PCR amplification or hybridization capture technology. Subsequently, high-throughput multi-pathogen parallel testing, grounded in NGS, is performed.

tNGS Technology – CD GenomicsCD Genomics tNGS Technology

Multiple research endeavors have consistently showcased the superior cost-effectiveness of targeted Next-Generation Sequencing (tNGS) over prevailing pathogen detection methods. tNGS exhibits heightened sensitivity, significantly minimizing the occurrences of both "false-positive" and "false-negative" outcomes in contrast to the established "gold standard" in pathogen diagnosis, as underscored by the influential Flurin study. Notably, the Flurin study distinctly revealed that tNGS outcomes outperformed the conventional culture-based test, exhibiting heightened sensitivity and a marked reduction in the incidence of false-positive and false-negative results.

Results of both tests in the periprosthetic joint infection group.Results of both tests in the periprosthetic joint infection group. (Flurin et al., 2021)

Workflow of Multiplex PCR Amplification

Workflow of Hybridization Capture Technology

We kick-start the process with DNA/RNA extraction, a pivotal step that lays the foundation for accurate analysis.

The journey continues with NGS library preparation. This involves cDNA synthesis and NGS Prep, collectively enhancing the genetic material for subsequent analysis. Our optimized methods reduce the preparation time to approximately 5 hours, ensuring a swift transition to the next stage.

Here, we delve into the heart of precision—target enrichment. Using cutting-edge hybridization capture techniques, we focus on amplifying the specific genetic regions of interest. While competitors often take up to 16 hours for this step, our streamlined approach slashes this time to around 4 hours. Portions covered through the Complete Kit are conveniently highlighted in red, keeping you informed at every stage.

Sequencing is where the magic happens. We harness the power of Illumina Sequencers, ensuring compatibility across platforms. The duration of this step depends on the read length, allowing for flexibility in achieving your desired results.

Completing the workflow, our dedicated team of experts engages in meticulous data analysis. In approximately 3 hours, we generate a comprehensive Virus Data Report—a valuable resource that distills complex data into actionable insights.


Empowering Our Clients Across Varied Applications

Our Pathogen Targeted NGS Service has demonstrated its prowess, proving to be a catalyst for progress, innovation, and effective solutions.


  1. Flurin, Laure, et al. "Targeted next generation sequencing for elbow periprosthetic joint infection diagnosis." Diagnostic Microbiology and Infectious Disease 101.2 (2021): 115448.

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