Details
| Product Infomation | |
|---|---|
| Product Description: | The CD Colorectal Cancer Gene RT-PCR Panel uses allele-specific primers to detect mutations in KRAS, NRAS, BRAF, PIK3CA, and AKT1 in multiplex PCR reactions. |
| Features: | Gene Exon Amino Acid Change Nucleotide Change Cosmic ID Reaction No. KRAS 2 G12A c.35G>C 522 1 G12D c.35G>A 521 1 G12R c.34G>C 518 1 G12C c.34G>T 516 1 G12S c.34G>A 517 1 G12V c.35G>T 520 1 G13D c.38G>A 532 1 3 A59T c.175G>A 546 6 A59E c.176C>A 547 6 A59G c.176C>G 28518 6 Q61H c.183A>C 554 4 Q61H c.183A>T 555 4 Q61L c.182A>T 553 4 Q61R c.182A>G 552 4 4 K117N c.351A>C 19940 1 K117N c.351A>T 28519 1 K117R c.350A>G 4696722 1 K117E c.349A>G – 1 A146T c.436G>A 19404 5 A146P c.436G>C 19905 5 A146V c.437C>T 19900 5 NRAS 2 G12D c.35G>A 564 2 G12S c.34G>A 563 2 G12C c.34G>T 562 2 G13R c.37G>C 569 2 G13V c.38G>T 574 2 3 A59T c.175G>A 578 6 A59D c.176C>A 253327 6 Q61K c.181C>A 580 3 Q61L c.182A>T 583 3 Q61R c.182A>G 584 3 Q61H c.183A>C 586 3 Q61H c.183A>T 585 3 4 K117R c.350A>G – 2 A146T c.436G>A 27174 5 BRAF 15 V600E c.1799T>A 476 3 V600E2 c.1799_1800TG>AA – 3 V600D c.1799_1800TG>AT 477 3 V600K c.1798_1799GT>AA 473 3 PIK3CA 9 E542K c.1624G>A 760 4 E545K c.1633G>A 763 4 E545Q c.1633G>C 27133 4 20 H1047R c.3140A>G 775 4 H1047L c.3140A>T 776 4 AKT1 4 E17K c.49G>A 33765 6 |
| Sequencing Platform: | Real-time PCR |
| Storage: | Store in < -20℃ freezer. Repeated thawing and freezing should be avoided. |
| Method: | Real-time qualitative PCR |
| Equipment Required: | Real-time PCR instrument (FAM, ROX, CY5 and VIC) |
| Required Reagents: | DNA extraction kit, pipettors and filter tips |
| ShippingCondition: | Dry ice |
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