Details
| Product Infomation | |
|---|---|
| Product Description: | The kit is based on real-time PCR technology to detect sheep authentication. |
| Features: | Features: 1. Highly sensitive and specific authentication of ingredients 2. Single, streamlined protocol for all targets 3. Dedicated DNA isolation kit available 4. PCR run time of 82 minutes on the QIAGEN Rotor-Gene Q 5. Full license for PCR without additional costs Kit components: 1. PCR Assay Sheep 2. Internal Control 3. Positive Control 4. Multiplex PCR Master Mix 5. QuantiTect Nucleic Acid Dilution Buffer 6. RNase-Free Water, 7. 50× ROX Dye Solution Cross-reactivity result: 1. Cattle – 2. Cod – 3. Water buffalo – 4. Salmon – 5. Goat – 6. Rabbit – 7. Deer – 8. Corn – 9. Turkey – 10. Soy – 11. Chicken – Procedure:l 1. DNA is extracted from a sample of food or animal feed using a dedicated sample preparation kit. QIAGEN's DNeasy Food Kit is suitable for a whole range of food matrices, including those with high inhibitor contents. 2. The isolated, pure DNA is assayed using a streamlined real-time PCR protocol that returns results in 82 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers. 3. The results are displayed as easy-to-interpret PCR curves. Principle: 1. Authenticating ingredients in food testing using real-time PCR is based on the amplification of a specific region of the relevant target genome. The amplified product is detected using target-specific fluorescent probes that bind to the amplified product. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes after the PCR run. The detection of target DNA confirms ingredient authenticity or prevents food fraud, ethical issues or health concerns. 2. PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes"). Tested DNA amplified Meaning： 1. Sample is positive for the target DNA 2. Sample is negative for the target DNA 3. PCR failed due to inhibition |
| Species: | Sheep |
| Sample Type: | Food and animal feed ingredients |
| Application: | This ingredient identification test kit is based on real-time PCR and is used to detect DNA fragments from specific animal sheep in food, animal feed, and pharmaceutical products. |
| Method: | Real-time PCR |
| Equipment Required: | Real-time PCR Instrument |
| ShippingCondition: | Dry ice |
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