Details
Product Infomation | |
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Product Description: | CD Tumor SNV 5% gDNA Standard II is a highly characterized, biologically relevant quality control material covering multiple genetic variants, including 10 SNVs, 2 insertion mutations and 1 deletion mutation and 1 fusion-associated variant validated by ddPCR. |
Features: | Quality Standards (1)Allele Frequency (AF) Genotype is tesed by Droplet Digital PCR, the acceptance criteria as follows: AF=0%, acceptable range ≤ 0.1%; AF < 5%, acceptable margin of error = ± 30%; 5% ≤ AF < 10%, acceptable margin of error = ± 20%; 10%≤AF, acceptable error range = ±10%. (2)Integrity is tesed by Agarose gel electrophoresis, the acceptance criteria as follows: Bright band of high molecular weight. SNV: BRAF V600E,EGFR L858R,EGFR T790M,EGFR G719A,KRAS G12C,KRAS G13D,NRAS Q61R,PIK3CA H1047R,PIK3CA E545K, FGFR3 Y375C. Deletion: EGFR ΔE746_A750del, MET Exon 14 Skipping. Insertion: EGFR A767_V769dup,ERBB2 A775_G776insYVMA. |
Method: | Quality Control Method: ddPCR |
Concentration: | 30 ng/μL |
Application: | CD Tumor SNV 5% gDNA Standard II is used to evaluate the performance of the NGS process and to help revise and monitor your protocols, protocols and bioinformatics processes for a range of subsequent applications. |
Preservation Solvent: | Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0 |
Storage: | Store at 2-8℃, 36 months. |
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