Details
| Product Infomation | |
|---|---|
| Product Description: | CD Tumor SNV 5% FFPE Standard is a highly characterized, biologically relevant FFPE quality control material. This standard covers multiple genes and contains 7 SNVs and 1 deletion mutation site validated by ddPCR. |
| Features: | Quality Standards (1)Fusion gene DNA mutation frequency is tesed by Droplet Digital PCR, the acceptance criteria as follows： AF=0%, acceptable range ≤0.1% 0＜AF＜1%, acceptable error range = ±40% 1% ≤ AF <5%, acceptable error range = ±30% 5% ≤ AF ≤ 20%, acceptable margin of error = ± 20% Copy number variation <5, acceptable error range = ±40% 5 ≤ copy number variation <10, acceptable error range = ±30% Copy number variation ≥10, acceptable error range = ±20% (2)Fusion gene RNA expression is tesed by End-Point RT-PCR. SNV: EGFR L858R, EGFR T790M, KRAS A146T, KRAS G12D, NRAS Q61K, PIK3CA E545K. Deletion: EGFR ΔE746_A750. |
| Method: | Quality Control Method: ddPCR |
| Concentration: | DNA extraction amount ≥400 ng/volume |
| Application: | CD Tumor SNV 5% FFPE Standard is used to evaluate each step of the entire process from sample extraction to quantification, helping to revise and monitor your experimental protocols, operational specifications and bioinformatics processes, etc., and to perform a range of subsequent applications. |
| Storage: | Store at 2-8℃, 48 months. |
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