Details
| Product Infomation | |
|---|---|
| Product Description: | CD 5% EGFR △E746-A750 FFPE DNA Standard is a highly characterized, biologically relevant FFPE quality control material covering the EGFR ΔE746-A750(6223) lung cancer hotspot single nucleotide mutation |
| Features: | Quality Standards (1)Fusion gene DNA mutation frequency is tesed by Droplet Digital PCR, the acceptance criteria as follows： AF=0%, acceptable range ≤0.1% 0＜AF＜1%, acceptable error range = ±40% 1% ≤ AF <5%, acceptable error range = ±30% 5% ≤ AF ≤ 20%, acceptable margin of error = ± 20% 20% ＜ AF, acceptable margin of error = ± 10% Copy number variation <5, acceptable error range = ±40% 5 ≤ copy number variation <10, acceptable error range = ±30% Copy number variation ≥10, acceptable error range = ±20% (2)Fusion gene RNA expression is tesed by End-Point RT-PCR. Deletion: EGFR △E746-A750. |
| Method: | Quality Control Method: ddPCR |
| Concentration: | DNA extraction amount ≥400 ng/volume |
| Application: | CD 5% EGFR △E746-A750 FFPE DNA Standard is used to evaluate the entire qPCR and Sanger sequencing process from sample extraction to quantification, to help revise and monitor your protocols, protocols and bioinformatics processes, and for a range of subsequent applications. |
| Storage: | Store at 2-8℃, 48 months. |
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