Details
| Product Infomation | |
|---|---|
| Product Description: | Enterocytozoon hepatopenaei (EHP) is a microsporidian parasite that infects the hepatopancreatic cells of cultured shrimp species, including Pacific white shrimp and black tiger shrimp. This pathogen was first identified in Thailand in 2009 and has since been reported in several other countries, including China, Vietnam, Malaysia, and Indonesia. EHP can cause a reduction in shrimp growth and survival rates, leading to significant economic losses in the shrimp aquaculture industry. Effective prevention and control measures are necessary to mitigate the impact of this pathogen on the industry. The kit is based on real-time PCR technology to detect EHP. |
| Features: | Components: 1. Probe Master Mix 2. EHP PP Mix 3. Endogenous PP Mix 4. EHP Positive control 5. Water, PCR grade 1 Specificity: EHP primer and probe have been designed for the specific and exclusive in vitro detection of EHP. The target sequence is highly conserved and sequences in this kit have 100% homology with a broad range of relevant reference sequences based on a comprehensive bioinformatics analysis. Dynamic linear range: The linear range was evaluated by analyzing a logarithmic dilution series of DNA concentrations ranging from 1.00E+09 to 1.00E+00 copies/μL. At least six replicates per dilution were analyzed. The linear range is 1.00E+09 to 1.00E+00 copies/ µL. Analytical sensitivity: The analytical sensitivity is defined as the concentration of DNA molecules (copies/μL) that can be detected with a positivity rate of 95%. The analytical sensitivity was determined by analysis of dilution series of quantified EHP specific DNA from 0.001 copies to 10 copies/ µL in triplicates. Under optimal PCR conditions, the analytical sensitivity is 0.25 copies per micro liter. |
| Species: | Enterocytozoon hepatopenaei (EHP) |
| Storage: | The kit is shipped on gel ice. Upon arrival, all components should be stored in -20℃. They are stable until the expiration date stated on the label. Repeated thawing and freezing should be avoided, as this might affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Storage at 2 to 8℃ should not exceed a period of 5 hours. |
| Method: | Real-time PCR |
| Equipment Required: | Agilent, Bio-Rad, Roche Lightcycler-96, RocheZ480/Cobas-480, Applied Bio systems [ABI], Thermo-Piko-Real, Rotor gene 5/6plex, Alta-96, Cepheid Real time PCR machines. |
| ShippingCondition: | Dry ice |
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