Details
| Product Infomation | |
|---|---|
| Product Description: | Early Mortality Syndrome (EMS), also known as Acute Hepatopancreatic Necrosis Disease (AHPND), is a devastating disease that affects farmed shrimp species, including Pacific white shrimp and black tiger shrimp. EMS was first reported in China in 2009 and has since spread to other regions, including Southeast Asia, Latin America, and the United States. The disease is caused by a toxin-producing strain of the bacterium Vibrio parahaemolyticus, which can colonize the shrimp's digestive system and cause massive destruction of the hepatopancreas. EMS can cause significant economic losses in the shrimp aquaculture industry. The kit is based on real-time PCR technology to detect Early Mortality Syndrome. |
| Features: | Kit components: 1. Probe Master Mix 2. EMS/Endogenous PP Mix 3. EMS Positive control 4. Water, PCR grade 1 Specificity: Vibrio parahaemolyticus primer and probe have been designed for the specific and exclusive in vitro detection of Vibrio parahaemolyticus plasmid harbouring toxin gene. The target sequence is highly conserved and sequences in this kit have 100% homology with a broad range of relevant reference sequences based on a comprehensive bioinformatics analysis. Dynamic linear range: The linear range was evaluated by analyzing a logarithmic dilution series of DNA concentrations ranging from 1.00E+09 to 1.00E+00 copies/μL. At least six replicates per dilution were analyzed. The linear range is 1.00E+09 to 1.00E+00 copies/ µL. Analytical sensitivity: The analytical sensitivity is defined as the concentration of DNA molecules (copies/μL) that can be detected with a positivity rate of 95%. The analytical sensitivity was determined by analysis of dilution series of quantified EMS specific DNA from 0.001copies to 10copies/ µL in triplicates. Under optimal PCR conditions, the analytical sensitivity is 0.25 copies per micro liter. |
| Species: | Early Mortality Syndrome (EMS) |
| Storage: | The kit is shipped on gel ice. Upon arrival, all components should be stored in -20℃. They are stable until the expiration date stated on the label. Repeated thawing and freezing should be avoided, as this might affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Storage at 2 to 8℃ should not exceed a period of 5 hours. |
| Method: | Real-time PCR |
| Equipment Required: | 1. Real-time PCR instrument having FAM & HEX channels 2. Automatic Nucleic acid extraction system or spin column based purification kit for the purification of nucleic acids Desktop centrifuge having 13000rpm or above with a rotor for 1. 5/2 mL reaction tubes 3. Centrifuge with a rotor for PCR strips/tubes and 96 well plates 4. Optical cap qPCR tubes or strips or 96 wells 5. Micro Pipettes (variables) 6. Micro Pipette tips with filters (disposable) 7. Powder-free gloves (disposable) |
| ShippingCondition: | Dry ice |
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